Quantifiable deliverables: For the Consortium

 

A. Scientific

 

Moisture stress, temperature, salinity and anoxia tolerance: Microbes

 

S.No.

Activity

I Year

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Deliverables

Output

Outcome

1.

Selection of different extreme environments for survey and collection of soil, water and sediment samples. The sites proposed to be surveyed are with regards to extreme conditions of

a)   Salinity/Sodicity/Acidity

b)   Moisture

c)    Temperature

d)   Submergence

Sample collection from selected extreme environments

Soil, water, sediment samples

Sample collection from selected extreme environments

Soil, water, sediment samples

 

 

 

2.

Analysis of diversity of predominant genera from various extreme environments and sequencing of rDNA genes for identification of selected isolates/strains.

Isolation of microbes/fungi/phytoplankton & zooplankton resistant to abiotic stress

Information on microbes/fungi/phytoplankton & zooplankton resistant to abiotic stress tolerance

Isolation of microbes/fungi/phytoplankton & zooplankton resistant to abiotic stress

Information on microbes/fungi/phytoplankton & zooplankton resistant to abiotic stress tolerance

 

 

Information on microbes/fungi/phytoplankton & zooplankton resistant to abiotic stress tolerance

Publication

3.

Specific primers for known genes will be used in PCR to identify and isolate candidate genes from microbes

Isolation and identification of genes responsible for abiotic stress in microbes/fungi/phytoplankton & zooplankton

Candidate genes for abiotic stress tolerance

Isolation and identification of genes responsible for abiotic stress in microbes

Candidate genes for abiotic stress tolerance

 

 

Candidate genes for development of transgenic plants.

Publication

4.

Construction of genomic library for suitable vector and screening of clones for identification of novel genes

 

 

Novel genes from abiotic stress tolerance from microbes

Abiotic stress tolerance from microbes

Novel genes from abiotic stress tolerance from microbes

Abiotic stress tolerance from microbes

Candidate gene for development of transgenic plants.

Publication

5.

Transposon mutagenesis of the selected strains for screening and isolation of the mutants of the interest and identification of the genes disrupted by transposon insertion in the mutants by cloning and sequence analysis of transposon flanking sequences

 

 

Microbe sensitive to abiotic stress

Functional validation of novel genes for abiotic stress

Microbe sensitive to abiotic stress

Functional validation of novel genes for abiotic stress

Candidate gene for development of transgenic plants.

 

Publication. Final report submission

6.

Metagenomic library construction and the sequence based screening for novel genes for candidate traits.

Isolation of abiotic stress tolerant genes from unculturable microbes.

Information about stress resistant genes from unculturable microbes.

Isolation of abiotic stress tolerant genes from unculturable microbes.

Information about stress resistant genes from unculturable microbes.

Isolation of abiotic stress tolerant genes from unculturable microbes.

Information about stress resistant genes from unculturable microbes.

Candidate gene for development of transgenic plants.

 

Publication. Final report submission

7.

Bioinformatics analysis of the identified gene and the encoded proteins and putative biochemical pathways responsible for the stress tolerance

Analysis of sequence data through software

Authentication of sequence information

Analysis of sequence data through software

Authentication of sequence information

Analysis of sequence data through software

Authentication of sequence information

Submission of gene sequences

 

 

Moisture stress, temperature, salinity, acidity and submergence tolerance: Rice

 

S.No

Activity

 

II Year

III Year

Deliverables

Outputs

Deliverables

Output

Outcome

1.

Identification of core collection of rice that would represent total diversity

Core collection of rice

Designation of core in rice

 

 

Enhanced utilization of germplasm

2.

Identification of mini-core collection of rice.

Characterization of core collections. mini-core collection of rice

Diversity in core. Designation of mini-core in rice

 

 

Value addition to germplasm

3.

Generation of phenotypic information for the mini-core collection

 

 

Validation of mini-core collection. Characterization of mini-core for target traits

National mini-core collection in rice. Accessions with specific traits

enhanced utilization of germplasm, Donor accessions for target traits

4.

Identification of candidate genes through differential expression analysis for selected traits

 

 

A set of differentially expressing genes in rice under stress

Candidate genes for stress tolerance

Candidate genes based marker for stress tolerance

5.

Selection of SNPs and SSRs for whole genome and candidate gene based genotyping

 

 

SSR and SNP markers well distributed in the genome

Markers for whole genome genotyping assay

Robust genotypic information based on sequence based markers

6.

Generation of SSR and SNP database for the mini-core collection

 

 

Molecular profiles of core accessions

Genotypic information for the whole genome

Protection of value added germplasm

7.

Allele mining for the selected known/candidate genes

 

 

Finding new allelic variants in germplasm

Understanding haplotype structure in selected gene region and their relations with target traits

New allele for stress tolerance for genetic improvement of rice

8.

Association mapping and identification of new genes/QTLs for the target traits

 

 

Identification of accessions and marker tags

Identification of genes for target traits

New genes for deployment in rice breeding

9.

Market assisted backcross transfer of useful genes/QTLs

 

 

Transfer of identified gene/QTLs for stress tolerance to desirable agronomic backgrounds

New lines with stress tolerance

Genetic improvement of rice for stress tolerance

10.

Fine mapping of the selected QTLs

 

 

Finding markers physically and genetically closer to the mapped genes

Marker tags for MAS

Tightly linked/gene-based markers for traits

11.

Cloning and characterization of orthologs of new/candidate genes from bajra

 

 

Identifying orthologs of stress tolerance genes/candidates in bajra

New set of drought tolerance genes from bajra

Stress tolerant orthologs for genetic improvement of rice

 

Moisture stress tolerance: Maize

 

S.No.

Activity

I Year

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Deliverables

Output

Outcome

1.

Collection of relevant germplasm for the target traits; including targeted exploration in the country

A core panel of atleast 300 genotypes for the each of the targeted trait, including atleast 50 landraces accessions from relevant species

Assembly of valuable genetic resources for basic, strategic and applied research

Sample collection from selected extreme environments

Soil, water, sediment samples

 

 

Documentation of genetic resources relevant for improving drought stress tolerance

2.

Phenotyping of ~300 genotypes under drought stress at multiple locations

(New Delhi; uchani; Hawalbagh)

Precise phenotyping of at least 100 genotypes per year for drought stress at three locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

Precise phenotyping of at least 100 genotypes per year for drought stress at three locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

Precise phenotyping for drought stress locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

Cataloguing of drought stress tolerant maize germplasm (inbreds and landraces) relevant to the Indian conditions.

3.

Intensive molecular characterization of 96 selected genotypes using genomic and genic SSRs and SNP markers

 

 

 

 

Molecular profiling of the 96 selected genotypes using genomic and genic SSRs and SNP markers

Profiles and database based on genomic and genic SSR and SNPs on phenotypically contrasting genotypes

Molecular database of a core set of drought tolerant maize genotypes

4.

Allele mining for 10 selected genes associated with drought stress tolerance

 

 

 

 

Primer design, sequence analysis and/or SNP analysis of selected genotypes for 10 candidate genes associated with drought stress tolerance in maize

Identification of specific allele variants in candidate genes for drought stress tolerance

Understanding of allelic variation for key candidate genes influencing drought stress tolerance

5.

SNP and haplotype pattern mining based on sequence analyses of candidate genes

 

 

 

 

Computational analysis of sequence data for SNP and haplotype pattern mining in candidate genes

Detection of a series of SNPs and haplotype in candidate genes

Basic understanding of linkage disequilibrium, SNP and haplotype variation for important genes influencing drought stress tolerance

6.

Association genetic analysis for identification of marker-trait associations

 

 

 

 

Integrated analysis of genotypic and phenotypic datasets for the target trait using association mapping

A set of SSR and SNP markers associated with drought stress tolerance, potentially useful for MAS

Genes and molecular markers that can be potentially deployed in molecular breeding for drought stress tolerance

 

 

Excess water stress tolerance: Maize

 

S.No.

Activity

I Year

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Deliverables

Output

Outcome

1.

Collection of relevant germplasm for the target traits; including targeted exploration in the country

A core panel of atleast 300 genotypes for the each of the targeted trait, including atleast 50 landraces accessions from relevant species

Assembly of valuable genetic resources for basic, strategic and applied research

 

 

 

 

Documentation of genetic resources relevant for improving drought stress tolerance

2.

Phenotyping of ~300 genotypes under drought stress at multiple locations

(New Delhi; Uchani; Hawalbagh)

Precise phenotyping of at least 100 genotypes per year for drought stress at three locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

Precise phenotyping of at least 100 genotypes per year for drought stress at three locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

Precise phenotyping for drought stress locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

Cataloguing of drought stress tolerant maize germplasm (inbreds and landraces) relevant to the Indian conditions.

3.

Intensive molecular characterization of 96 selected genotypes using genomic and genic SSRs and SNP markers

 

 

 

 

Molecular profiling of the 96 selected genotypes using genomic and genic SSRs and SNP markers

Profiles and database based on genomic and genic SSR and SNPs on phenotypically contrasting genotypes

Molecular database of a core set of drought tolerant maize genotypes

4.

Transcriptome profiling for excess water stress tolerance and identification of differentially expressed genes

 

 

 

 

Microarray analysis of excess water using selected highly contrasting genotypes based on experiments at multiple locations.

Identification of significant differentially expressed genes under excess water stress in the Indian context

Detection of important genes and pathways associated with excess water stress tolerance in crop plants

5.

Allele mining for 6 selected candidate genes associated with excess water stress tolerance

 

 

 

 

Primer design, sequence analysis and/or SNP analysis of selected genotypes for 6 candidate genes associated with excess water stress tolerance

Identification of specific allelic variation in candidate genes for

excess water stress tolerance

Understanding of allelic variation for key candidate genes influencing excess water stress tolerance

6.

SNP and haplotype pattern mining based on sequence analyses of candidate genes

 

 

 

 

Computational analysis of sequence data for SNP and haplotype pattern mining in candidate genes

Detection of a series of SNPs and haplotypes in candidate genes

Basic understanding of linkage disequilibrium, SNP and haplotype variations for important genes influencing excess water stress tolerance

7.

Association genetic analysis for identification of marker-trait associations

 

 

 

 

Integrated analysis of genotypic and phenotypic datasets for the target trait using association mapping

A set of SSR and SNP marker associated with excess water stress tolerance, potentially useful for MAS

Genes and molecular markers that can be potentially deployed in molecular breeding for influencing excess water stress tolerance

 

Moisture Stress tolerance: Sorghum

 

S. No.

Activity

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Outcome

1.

Validation of the resistant and susceptible genotypes or Sorghum

Core collection of Sorghum genotypes

Designation of core in Sorghum

 

 

Enhanced utilization of germplasm

2.

Identification of candidate genes through differential expression analysis for drought traits

Precise phenotyping of at least 100 genotypes per year for drought stress at three locations based on appropriate morph-physiological traits

Identification of scores of tolerance to drought stress suitable to different agro-ecologists

A set of differentially expressing genes in Sorghum under water stress

Candidate genes for stress tolerance

Candidate gene based new alleles and markers for stress tolerance

 

Moisture Stress tolerance : Cucurbitis, Lathyrus and Ziziphus

 

S. No.

Activity

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Outcome

1.

Identification of core and reference collection of target crops using eco-geographical data.

Core collection of cucumber (300 acc.) and muskmelon (200 acc.)

Designation of cores in cucumber and melons

 

 

Enhanced utilization of germplasm

2.

Multi-location evaluation of the core collection foe well defined descriptors

 

 

Characterization of core collections of 15 morphological traits

Diversity in core

Value addition to germplasm

3.

Refinement of core collection based on evaluation data and additional information on genetic diversity

 

 

Validation of core collection

National core collection in cucumber and melons

Enhanced utilization of germplasm

4.

Phenotyping of the core collection for target traits under standard and controlled conditions in three locations.

 

 

Characterization of core for target traits for WUE, moisture stress, heat tolerance, chlorophyll content etc.

Accessions with specific traits

Enhanced utilization of germplasm

5.

Genotyping of core collection using AFLP, SSR, and SNP markers selected to represent the whole genome.

 

 

 

Molecular profiles of core accessions

Protection of value added germplasm

6.

Association analysis to identify genes for target traits for identification of accessions and marker tags

 

 

 

Identification of accession and marker tags

Donor accession for target traits

7.

Fine mapping of selected genes for use in marker assisted transfer of the traits

 

 

 

Tagging of target genes

Marker tags for MAS

8.

Cross validation of results among the partners for specific trait identified

 

 

 

Identification of genes and allele target traits.

Allelic variation for deployment

9.

Biochemical evaluation for quality traits to define the variations identified

 

 

 

Donor for quality traits

Allelic variants for quality traits

 

Heat tolerance: Moth bean

 

S. No.

Activity

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Outcome

1.

Analysis of eco-geographical data and identification of core collection in moth bean including wild species

Core collection of moth bean (350 acc.)

Designation of cores in moth beans

 

 

Enhanced utilization of germplasm

2.

Screening and classification of core collection for heat tolerance

 

 

Characterization of core collections

Diversity in core

Value addition to germplasm

3.

Isolation cloning of genes for heat tolerance based on homology

 

 

Identification and cloning of genes for heat tolerance

A set of cloned genes involved in heat tolerance mechanism from moth bean

Enhanced utilization of germplasm

4.

Validation of isolated genes through transformation in moth bean/Arabidopsis

 

 

Functional validation

A system to study genes further through expression analysis

Enhanced utilization of germplasm

5.

Identification of candidate genes through differential expression profiling

 

 

Information on heat stress induced genes

Information on candidate genes related to heat tolerance

Protection of value added germplasm

6.

Allele mining for the selected genes

 

 

Identification of elite germplasm lines

Novel alleles of genes for the target traits

Donor accessions for target traits

 

Heat and Cold tolerance: Camel and Goat

 

S.No.

Activity

I Year

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Deliverables

Output

Outcome

7.

Establishment of metabolic and biochemical parameters in goats

The various BMR parameters of goat

Basal metabolic and biochemical parameters profile in goats various climatic conditions

The various BMR parameters of goats under various climatic conditions shall be records.

Basal metabolic and biochemical parameters profiles in goats various climatic conditions.

The various BMR parameters of goats under various climatic conditions.

Basal metabolic and biochemical parameters profile in goats various climatic conditions.

BMR profile and various biochemical parameters of goats under normal and various climatic conditions established

8.

Establishment of metabolic and biochemical parameters in goats under artificially induced stress conditions

The various BMR parameters of goats under artificially induced stress conditions shall be recorded.

Basal metabolic and biochemical parameter profile in goats artificially induced stress conditions,

The various BMR parameters of goat under various artificially induced conditions shall be recorded.

Basal metabolic and biochemical parameters profile in goats various artificially induced stress conditions

The various BMR parameters of goat under various artificially induced conditions shall be recorded.

Basal metabolic and biochemical parameters profile in goats various artificially induced stress conditions

BMR profiles and various biochemical parameters of goats under artificially induced stress conditions established.

9.

Establishment of metabolic and biochemical parameters in Bactrian camel

The various BMR and biochemical parameters of Bactrian camel under normal conditions shall be recorded.

Basal metabolic and biochemical parameter profile in Bactrian camel various climatic conditions

The various BMR and biochemical parameters of Bactrian camel under various climatic conditions shall be recorded

Basal metabolic and biochemical parameters profile Bactrian camel various climatic conditions

The various BMR and biochemical parameters of Bactrian camel under various climatic conditions shall be recorded

Basal metabolic and biochemical parameters profile Bactrian camel various climatic conditions

BMR profiles and various biochemical parameters of goats under artificially induced stress conditions established.

10.

Establishment of metabolic and biochemical parameters in Dromedarian camel

The various BMR and biochemical parameters of Dromedarian camel under normal conditions shall be recorded

Basal metabolic and biochemical parameter profile in Dromedarian camel various climatic conditions

The various BMR and biochemical parameters of Dromedarian camel under various climatic conditions shall be recorded

Basal metabolic and biochemical parameters profile Dromedarian camel various climatic conditions

The various BMR and biochemical parameters of Dromedarian camel under various climatic conditions shall be recorded

Basal metabolic and biochemical parameters profile Dromedarian camel various climatic conditions

BMR profiles and various biochemical parameters of goats under artificially induced stress conditions established.

11.

Identification of variations in camel and goat for various candidate genes associated with thermoregulatory mechanisms in mammals in different geographical locations having extremes of temperature variations

Variations shall be recorded among the goats and camel species of different agro-climatic regions for the candidate genes associated with thermoregulatory mechanism

SNPs in the candidate genes

Genotypes of various goats and camel species under different agro-climatic regions

Population structure of goats and camel species based on the SNPs for the candidate genes involved in the thermoregulatory mechanisms

 

 

Goat and camel specific SNPs associated with adaptive trait and population structure of Indian goat and camel species.

12.

Transcriptome analysis of goats and camels

mRNA profile of camel and goat shall be carried out.

Transcriptome of goat and camel revealed.

mRNA quantification of goat and camel under various stress conditions

Changed profile of mRNA as an effect of stress response

Transcriptome analysis of goats and camels under various stress conditions

Under and over-expression of genes related to stress tolerance

Transcriptome profile for camel and goat established.

 

Salinity tolerance: Fishes

 

S. No.

Activity

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Outcome

1. Identification and characterization of genes for salinity stress in giant freshwater prawns. M. rosenbergii and Penaeus monodon

Construction of cDNA libraries by SSH and normalized techniques from wild and accimilized individuals.

Conversion of normalizes cDNAs to Illumina GA-sequencing ready libraries followed by IIlumina GA (Solexa) Sequencing,

Assembly sequences for custom bioinformatics.

Cloning normalized cDNA into pGEMT-easy vector.

Sequencing of full length cDNA by RACE.

Estimation of transcripts levels by Real-Time PCR

Report writing

SSH and normalized cDNA libraries

Sequences of genes expressed in response to saline stress

Annotated sequence data and clones

Knowledge of genes expressed in response to saline stress and their clones

Identification of novel genes responsive to saline stress from M. rosenbergii and P. monodon

2. Functional characterization of genes identified in P. monodon

Complete sequencing of three genes over-expressed in P. monodon acclimatized to 35 ppt and 1 gene over-expressed in 0 ppt. using RACE.

Transcript levels at different salinities in P. monodon gill and muscle tissue using Real Time PCR

Cloning of cDNA sequences an a constitutive His-tag expression vector and over-expression in a eukaryotic cell line.

Purification of the His-tagged protein using Ni column and raising antibodies in rabbit.

Sub-cellular localization of the proteins in gill and muscle using antibodies and tracing expression at different salinities.

Sequences of four genes known to be expressed in response to saline stress in P. monodon

Full-length cDNA clones of the genes and knowledge of their involvement in saline stress in P. monodon

Purified proteins that are important for saline adaptation Final report/Publications

Candidate gene based new alleles and markers for stress tolerance

Functional characterization and validation of four novel genes responsive to saline stress in P. monodon

Cold tolerance: Fish (Trout)

 

S.No.

Activity

I Year

II Year

III Year

 

 

 

Deliverables

Output

Deliverables

Output

Deliverables

Output

Outcome

1.

Collection of fish samples from different high altitude colder environments in J&K, Himachal Pradesh and Uttarakhand

Collection of fish samples from J&K, Himachal Pradesh and Uttarakhand

Samples from different sites will be available for gene prospecting

 

 

 

 

New trout sources for cold tolerance genes

2.

Molecular identification and sequence variation between the exotic and indigenous trout species for cold tolerant genes

PCR amplification for cold tolerant genes

Identified of amplified cold tolerant genes

 

 

 

 

Identification of amplified cold tolerant genes from different part of countries

3.

Construction of genomic library in suitable vector and screening of clones for identification of novel genes

 

 

Development of cDNA libraries

cDNA libraries

 

 

cDNA libraries for target gene isolation for cold tolerance

4.

Validation of gene expression

 

 

 

 

Validated expression patterns

A set of genes showing specific expression in trout under cold conditions

Genes for cold tolerance from trout

 

Anoxia Stress tolerance : Fish (catfish)

 

S. No.

Activity

II Year

 

 

 

Deliverables

Output

Outcome

1.

Identification and characterization for submergence resistance I Fishes, Clarias batrachus and Channa striatus

Characterization of genes involved in anoxia stress tolerance

Identification and quantification of differential gene regulation (up/down regulation)

Genome resources for allele mining and genetic manipulation for anoxia resistance

2.

Development of tissue specific ESTs through Transcriptime analysis in Fishes, Clarias batrachus and Channa sriatus

Total set of expressed genes in different tissues

Understanding of tissue specificity gene expressions

A detailed database of expression profile in different tissues in Fishes, to be used as reference for the future studies.

 

Statistical and Computational Genomics:

 

S. No.

Activity

I Year

II Year

III Year

 

 

Deliverables

Output

Deliverables

Output

Deliverables

Output

1.

Statistical and computational analysis of genomic data generated from plants, animals, and fish genetic resources

Extending statistical and computational analysis support to scientists working in genomics.

Development of centralized laboratory with software for analyzing high throughput genome sequence data

Extending statistical and computational analysis high throughput genome sequence data

Whole genome association analysis, Transmission disequilibrium testing, Microarray data analysis, etc, can be done at the laboratory

Completion of analyzing genome data generated under the consortiums

Provision of full fledge statistical and computational support to analyze genomic data.

2.

Development of algorithms for identified of features of splice site efficiency through comparative genomics

Development of databases on 5 splice site motifs across species

Development of centralized splice rack

Characterization of features of splice site regions across species through algorithmic approach

Users would be provided with tools to identify splice site features across the species

Tested and validated algorithms would be converted to software for easy usage

Assessment of splice site efficiency

3.

Training scientific personnel in the fields of statistical genomics and Bioinformatics

Completion of two training programmes with 20 participants each

Capacity building

Composition of two training programmes with 20 participants each

 

Composition of two training programmes with 20 participants each

Capacity building in statistical genomics and Bioinformatics

 

B.                                Physical

 

I.                 Procurement of goods/services/equipments/consultancy etc.

 

Objectives

Deliverables at the end of the 1st year

..

Deliverables at the end of the final year

Final Output

  1. Generation of genomic resources base to facilitate gene prospecting and allele mining
  2. Prospecting for new genes and alleles for abiotic stress tolerance (moisture stress, salinity and sodicity, soil acidity, adverse temperature and submergence/anoxia)
  3. Functional validation of the identified genes in model plant systems.
  4. Use of the identified genes/allele in genetic enhancement of target species

Procurement of equipments and phenotying facilities

 

Establishment of high throughput genotyping and phenotyping facilities

Efficient phenotyping and genotyping of germplasm

 

II Hiring Manpower

 

Objectives

Deliverables at the end of the 1st year

..

Deliverables at the end of the final year

Final Output

1.        Generation of genomic resource base to facilitate gene prospecting and allele mining

2.        Prospecting for new genes and allele for abiotic stress tolerance (moisture stress, salinity and sodicity, soil acidity, adverse temperature and submergence/anoxia)

3.        Functional validation of the identified genes in model plant systems

4.        Use of the identified genes/allele in genetic enhancements of target species

Recruitment of SRF/RAs

 

Trained manpower for allele mining

Human resource development in allele mining and bioprospecting

 

Deliverables in the above table are the defined periodical outputs in the achieving milestones and the final output

 

C.                               Financial

 

I Use of funds

 

 

Milestone End of the year 1 (%)

Deliverables End of the year 1 (Rs in Lakhs)

 

Milestone End of the year 2 (%)

Deliverables End of the year 2

(Rs in Lakhs)

Milestone End of the year 3 (%)

Deliverables End of the year 3

(Rs in Lakhs)

Milestone End of the final year (%)

Deliverables End of the Final year

(Rs in Lakh)

Expressed Output (% utilization)

 

Item-wise

Renovation/New works

100

270.50

-

-

-

-

-

-

100

Equipment, plant & Machinery

100

1652.29

-

-

-

-

-

-

100

Organizing Training/ Workshop

0.00

0.00

66.67

10.00

33.33

5.00

1.00

15.00

100

Foreign Training and visit

55.78

55.50

41.71

41.50

2.51

2.5

100

99.50

100

TA/DA

33.33

24.217

33.33

34.217

33.33

24.217

100

72.65

100

Review Workshop/Meetings

38.36

6.33

30.81

5.083

30.81

5.083

100

16.50

100

Contractual staff

(SRFs/RA)

33.33

159.095

33.33

159.095

33.33

159.095

100

477.284

100

Operational Contingencies

35.79

1040.07

37.09

1077.84

27.12

787.97

100

2905.88

100

Institutional Charges

35.78

72.868

37.02

75.382

27.20

55.388

100

203.639

100

 

II Audit completion (second year onwards): give dates by which audit report of the respective previous years accounts will be ready):

For all institutes the financial audit will be completed by June in every financial year

 

9.               Please give (third year of the sub-project onwards) the expected outcomes, for the project as a whole here and for each institutions separately as Annexure 7 in the following format.

 

For the Consortium

 

Outcome Indicator

First Year

Second Year

Third Year

1. Outstanding scientific problems solved

       Identification of core collection

       Confirmation of stress tolerant genotypes

       Characterized genetic stocks

       cDNA libraries of expressed sequences

       Value addition to the genetic resources

       New genetic stocks for improvement of target species

       Functional genes for stress tolerance

2. New methodology developed and the scientific benefits it provides

Development of procedure for evaluation of abiotic stress tolerance

Screening techniques for stress tolerance

Characterized genetic stocks

New SSRs, SNPs and other markers

       Association mapping technique

       Half sib technique

       Screening techniques

       Core/reference collection

       Facility for phenotypic stress tolerance

       High throughput genotypic facility

3. New frontier technology developed (including conceptually novel varieties, breeds, strains etc.)

 

       Haplotype structure

       Marker tags for tolerance to abiotic stresses in the target species

       Breeding lines, genetic stocks; new allelic variations in different target species

       Germplasm with new allelic variations for stress tolerance

4. New knowledge and skills acquired by the scientists

       Genetic control of target traits

       Expertise in screening and value addition to genetic resources in diverse crops

       Skills for exploitation of germplasm in crop improvement

       Structure of populations

       Distribution of allelic variation

       Trained human resources in bioprospecting and allele mining

5. Patents taken/applied for

 

 

New genetic stocks in the crops will be registered

New genes, alleles and technology to protected

6. Patents sold/ royalty arrangements made

 

 

To be negotiated